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GLP-1 agonist● Clinical trials

Semaglutide

Semaglutide is an FDA-approved GLP-1 receptor agonist marketed as Ozempic (type 2 diabetes, cardiovascular risk reduction) and Wegovy (chronic weight management), backed by large phase-3 trial programs (STEP, SUSTAIN).

Molecular weight
~4113.6 g/mol
g/mol
Half-life
~7 days (once-weekly dosing)
estimated
Form
Injectable solution (pre-filled pen, SubQ); oral tablet (Rybelsus) also FDA-approved
FDA status
FDA-approved (Rx-only) — Ozempic 2017, Wegovy 2021

Semaglutide reconstitution calculator

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Typical dosing

GoalRangeRouteFrequency
Initiation (GI tolerability)0.25 mgSubQ, abdomen/thigh/upper armOnce weekly, weeks 1-4 (not a therapeutic dose)
Titration step 10.5 mgSubQ weeklyWeeks 5-8
Titration step 21 mgSubQ weeklyWeeks 9-12
Titration step 3 (Wegovy only)1.7 mgSubQ weeklyWeeks 13-16
Maintenance — weight management (Wegovy)2.4 mgSubQ weeklyWeek 17 onward, ongoing
Maintenance — glycemic control (Ozempic)0.5-2 mgSubQ weeklyPer FDA label, after initial titration; max 2 mg/week

Ranges compiled from research literature — not a prescription.

How it works

Semaglutide is a synthetic analog of human glucagon-like peptide-1 (GLP-1), modified with a fatty-diacid side chain and amino-acid substitutions that confer resistance to DPP-4 degradation and reversible albumin binding, producing an elimination half-life of roughly one week that supports once-weekly dosing. As a GLP-1 receptor agonist, it binds GLP-1 receptors on pancreatic beta cells, the hypothalamus, and the gut.

Pharmacologically, semaglutide increases glucose-dependent insulin secretion, suppresses glucagon release, slows gastric emptying, and acts on hypothalamic appetite centers to reduce hunger and food intake. The combined effect on satiety and gastric emptying is the primary driver of weight loss, while the insulinotropic and glucagonostatic effects drive glycemic control in type 2 diabetes.

The evidence base is unusually large for a peptide drug. In the STEP 1 trial (1,961 adults with overweight/obesity, no diabetes), semaglutide 2.4 mg produced a mean 14.9% body-weight reduction versus 2.4% with placebo at 68 weeks, with 86.4% of treated participants losing at least 5% of body weight. STEP 1 extension data showed most of the lost weight was regained within a year of stopping treatment, underscoring that effects are dependent on continued use. In SUSTAIN-6 (3,297 adults with type 2 diabetes and high cardiovascular risk), semaglutide reduced the composite endpoint of cardiovascular death, nonfatal MI, or nonfatal stroke by 26% versus placebo (HR 0.74, 95% CI 0.58-0.95) over ~2 years, supporting the cardiovascular risk-reduction indication later added to the Ozempic label.

Because of this trial base, semaglutide carries full FDA approval and is legally available only by prescription in FDA-regulated, quality-controlled pen formulations (Ozempic, Wegovy) or tablets (Rybelsus). This is a meaningfully different risk profile from "research" or compounded semaglutide sold outside the pharmacy system, which is not FDA-approved, is not manufactured or tested under the same quality standards, and falls outside the dosing, monitoring, and pharmacovigilance framework established in the trials above.

Side effects & safety

  • Gastrointestinal effects are the most common adverse reactions: nausea (~44% vs 16% placebo), vomiting (~25% vs 6%), and diarrhea (~30% vs 16%) in Wegovy weight-management trials; these are the leading cause of discontinuation.
  • Boxed warning: in rodent studies semaglutide caused thyroid C-cell tumors; it is unknown whether this occurs in humans, so semaglutide is contraindicated in patients with a personal or family history of medullary thyroid carcinoma (MTC) or Multiple Endocrine Neoplasia syndrome type 2 (MEN 2).
  • Acute pancreatitis has been reported in patients treated with semaglutide and other GLP-1 receptor agonists; discontinue promptly if pancreatitis is suspected.
  • Increased risk of gallbladder disease (cholelithiasis, cholecystitis), likely related to rapid weight loss, has been observed in weight-management trials.
  • Contraindicated with hypersensitivity to semaglutide or any product component, in addition to the MTC/MEN 2 contraindication above; use with caution alongside insulin or sulfonylureas due to hypoglycemia risk, and monitor renal function and diabetic retinopathy status in patients with type 2 diabetes.
  • Legally requires a prescription, medical evaluation, and clinician-supervised dose titration and monitoring — it is not an over-the-counter or self-directed research chemical.

Research

Primary sources — PubMed.

  1. FDA. Highlights of Prescribing Information: OZEMPIC (semaglutide) injection. 2025. https://www.accessdata.fda.gov/drugsatfda_docs/label/2025/209637s025lbl.pdf
  2. FDA. Highlights of Prescribing Information: WEGOVY (semaglutide) injection. 2025. https://www.accessdata.fda.gov/drugsatfda_docs/label/2025/215256s024lbl.pdf
  3. Wilding JPH, et al. Once-Weekly Semaglutide in Adults with Overweight or Obesity (STEP 1). N Engl J Med. 2021. https://www.nejm.org/doi/full/10.1056/NEJMoa2032183
  4. Marso SP, et al. Semaglutide and Cardiovascular Outcomes in Patients with Type 2 Diabetes (SUSTAIN-6). N Engl J Med. 2016. https://www.nejm.org/doi/full/10.1056/NEJMoa1607141
  5. Wilding JPH, et al. Weight regain and cardiometabolic effects after withdrawal of semaglutide: The STEP 1 trial extension. Diabetes Obes Metab. 2022. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542252/
  6. Overgaard RV, et al. Clinical Pharmacokinetics of Semaglutide: A Systematic Review. Drug Des Devel Ther. 2021. https://www.dovepress.com/clinical-pharmacokinetics-of-semaglutide-a-systematic-review-peer-reviewed-fulltext-article-DDDT
  7. Husain M, et al. Cardiovascular Safety and Benefits of Semaglutide in Patients With Type 2 Diabetes: Findings From SUSTAIN 6 and PIONEER 6. Front Endocrinol. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039387/
  8. Garvey WT, et al. Two-year effects of semaglutide in adults with overweight or obesity: the STEP 5 trial. Nat Med. 2022. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556320/
⚠︎Semaglutide may be a research chemical not approved for human use in your country. This page is educational information only — not medical advice, and not an endorsement to use it.
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Semaglutide FAQ

In the STEP 1 trial, adults with overweight or obesity and no diabetes lost a mean of 14.9% of body weight on semaglutide 2.4 mg versus 2.4% on placebo over 68 weeks, and 86.4% of treated participants lost at least 5% of their body weight. Individual results vary considerably, and these figures come from trials that paired the drug with lifestyle counseling.
Most of the lost weight comes back. The STEP 1 trial extension followed participants after treatment withdrawal and found they regained the majority of their lost weight within a year, with cardiometabolic improvements reverting toward baseline as well. This is why semaglutide is framed as an ongoing chronic-weight-management therapy rather than a finite course of treatment.
About 16 weeks for the 2.4 mg weight-management dose. The label starts at 0.25 mg weekly for weeks 1-4, then steps to 0.5 mg, 1 mg, and 1.7 mg at four-week intervals, reaching 2.4 mg maintenance at week 17. This gradual titration exists specifically to limit nausea, vomiting, and diarrhea, which are the leading causes of discontinuation.
No. Ozempic, Wegovy, and Rybelsus are FDA-approved, quality-controlled products dispensed by prescription. Compounded or research-grade semaglutide is not FDA-approved, is not manufactured or tested under the same standards, and falls outside the dosing, monitoring, and pharmacovigilance framework established in the STEP and SUSTAIN trials. Identity, strength, and sterility are not independently verified.

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